A luciferase assay is a bioluminescent technique used to measure gene expression, promoter strength, or protein activity. It works by using the luciferase enzyme to catalyze the oxidation of a substrate called luciferin, which produces light. The intensity of the light emitted is directly proportional to the amount of luciferase enzyme, which in turn reflects the activity of the gene or promoter being studied. | Tsouryel Ben Israel